Abnormal Electrophoretic Mobility of a Creatine Kinase MM Isoenzyme
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چکیده
منابع مشابه
Abnormal electrophoretic mobility of a creatine kinase MM isoenzyme.
The routine determination of creatine kinase (CK; EC 2.7.3.2) isoenzymes has recently gained acceptance as a diagnostic tool in laboratory medicine (13). Electrophoreticaily three distinct CK isoenzymes can be separated (4, 5). These dimeric bands have been identified throughout the literature as MM, MB, and BB, and originate mainly from skeletal muscle, heart, and brain tissues, respectively. ...
متن کاملMetastable creatine kinase MM and complexes of creatine kinase BB and immunoglobulin G, "atypical" isoenzymes with similar electrophoretic mobility.
Metastable creatine kinase MM isoenzyme was isolated and partially purified from homogenates of myocardium and skeletal muscle by gradient elution on carboxymethyl cellulose. This variant isoenzyme migrated between the MM and MB isoenzymes on agarose electrophoresis, accounted for 3.5% of the total creatine kinase activity in each tissue, was not a macromolecule, and had stable electrophoretic ...
متن کاملFurther heterogeneity demonstrated for serum creatine kinase isoenzyme MM.
Serum creatine kinase (EC 2.1.3.2) isoenzyme MM was resolved by isoelectric focusing into a five-band pattern, a pattern that gradually changed after the onset of myocardial infarction. Similar changes were also demonstrated in patients undergoing coronary-bypass surgery. The evolution of two CK-MB sub-bands was studied in both cases. We found that three electrophoretic bands (CK-MM, pI 7.10; M...
متن کاملHeterogeneity of serum creatine kinase isoenzyme MM in myocardial infarction: clinical significance and post-synthetic conversion of "abnormal" sub-bands.
We investigated the "abnormal" sub-bands of serum creatine kinase (CK; EC 2.7.3.2) isoenzyme MM in acute myocardial infarction (AMI), using isoelectric focusing in polyacrylamide gels, and compared the patterns with those for non-AMI patients and for athletes with increased CK. The "abnormal" sub-bands a (pI 7.55), b (pI 7.35), c (pI 7.25), d (pI 7.05), e (pI 6.85), f (pI 6.72), g (pI 6.50), h ...
متن کاملCreatine kinase isoenzyme MB: discrepancy between immuno-inhibition and electrophoretic measurement in lyophilized proficiency samples.
Then theircontentswere assayedfor vitamins E and A. Our results all agreed, within theerror of the methods, showing that neither Venojet nor Vacutainer Tube stoppers of the types available in South Africa contain dyes or antioxidants interfering with the assay of vitamin E, in agreement with the work of Sinclair and Slattery (1), or with the assay of vitamin A. Althoughthese results indicate th...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1975
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/21.12.1837